The most important laboratory procedures in the National Reference Laboratory for Human Parasitic Diseases
Diagnostic laboratory procedures
Antibody detection
- Anti-Toxoplasma IgG ELISA
- Anti-Toxoplasma IgM ELISA
- Anti-Toxoplasma IgA ELISA
- Anti-Toxoplasma IgG avidity test by ELISA
- Anti-Toxoplasma IgG+IgM comparative (mother-child pairs) Western blot
- Anti-Toxocara IgG ELISA
- Anti-Toxocara IgG Western blot
- Anti-Echinococcus IgG ELISA
- Anti-Echinococcus indirect haemagglutination
- Anti-Echinococcus granulosus/multilocularis IgG Western blot
- Anti-Trichinella IgG ELISA
- Anti-Trichinella antibody detection by microprecipitation
- Anti-Trichinella IgG Western blot
- Anti-Entamoeba histolytica indirect haemagglutination
- Anti-Taenia solium cysticercosis IgG Western blot
- Anti-Leishmania IgG Western blot
- Anti-Schistosoma IgG Western blot
Microscopic examinations
- Direct examination of wet mount preparations
- Concentration of protozoan cysts
- Detection of Cryptosporidium parvum by Kinyoun staining
- Concentration of helminth ova by flotation
- Concentration of helminth ova by sedimentation
- Concentration of larvae
- Examination of anorectal scrape
- Examination of specimens suspicious for helminthes, identification by morphology
- Microscopic examination of the content of Echinococcus cysts
- Homogenization and microscopic examination of sputum
- Detection of Plasmodium spp. in Giemsa-stained preparations
- Detection of Trypanosoma spp. in native and stained preparations
- Detection of microfilariae in blood by Knott concentration technique or in skin specimens
- Morphologic and histopathologic examination of Dirofilaria spp.
- Microscopic examination of "free-living" amoebae
Antigen detection
- Giardia intestinalis antigen detection in stool by ELISA
- Detection of Entamoeba histolytica-specific lectin-antigen in stool by ELISA
- Detection of Cryptosporidium parvum antigens by immunchromatography or ELISA
- Detection of Plasmodium antigens by immunochromatography
Culture
- Culture of protozoan in Boeck-Drbohlav biphasic egg-slant medium, supplemented with bovine serum
- Culture of Trichomonas vaginalis in CPLM medium
- Culture of "free-living" amoebae on Page-agar plates covered with bacteria
Molecular methods
- Toxoplasma gondii B1 gene specific real-time PCR
- Toxoplasma gondii B1 gene specific nested PCR
- Toxoplasma gondii SAG2 gene specific PCR for genotyping the strains after sequential analysis for epidemiological purposes
- Detection of Plasmodium genus specific DNA by real-time PCR
- Detection of Plasmodium species specific DNA by seminested multiplex PCR
- Giardia intestinalis 18S rDNA specific PCR for genotyping after sequential analysis for epidemiological purposes
- Entamoeba histolytica 18S rDNS gene specific PCR
Maintenance/culture of laboratory control strains
- Maintenance of T.gondii strain by culture in mice
- Maintenance of a Trichinella spiralis strain by culture in mice
- Maintenance of Trichomonas isolates by culture in CPLM medium
- Maintenance of Blastocystis hominis isolates by culture in egg-slant medium
- Maintenance of "free-living" amoebae (Acanthamoeba sp., Naegleria fowleri) on Page-agar plates covered with bacteria
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